@DagobertIX @chris_komatsu @RandPaul <<FCS insertion is CTCCTCGGCGGG. The reverse complement is 5′‑ CCCGCCGAGGAG ‑3′. GCCGAG is the NmeAIII recognition site, but only visible on the antisense strand, not the sense strand.>>
@tony_vandongen noted something like this when raised originally.